This study aims to determine the validity, reliability, level of difficulty, and discrimination power of an artificial intelligence (AI)-generated collection of biology questions for higher education. Students’ responses to AI-generated questions are also presented in this study. A sample of 272 students was selected using a random sampling technique to answer a series of multiple-choice questions and complete a questionnaire. Based on the research findings, 20 of the 21 questions generated by ChatGPT AI are valid. Cronbach’s alpha coefficient was determined to be 0.65 (fairly reliable) for the twenty valid questions. Based on student responses to questions generated by ChatGPT’s AI, it was determined that 79% of students indicated that the AI-generated questions were relevant to the class subject. 72% of students reported that the clarity of AI-generated questions was acceptable. 73% of students reported that the accuracy of AI-generated questions was good.
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